A New Approach for Developing Spectrophotometric Determination of Phenylephrine Drug in Pure, Pharmaceutics and Serum Samples Using Sodium Periodate as Oxidizing Agent via a Green Method of CFIA/Merging Zone Technique

The research involved a rapid, automated and highly accurate developed CFIA/MZ technique for estimation of phenylephrine hydrochloride (PHE) in pure, dosage forms biological sample. This method is based on oxidative coupling reaction 2,4-dinitrophenylhydrazine (DNPH) with PHE existence sodium periodate as oxidizing agent alkaline medium to form red colored product at ʎmax )520 nm (. A flow rate 4.3 mL.min-1 using distilled water carrier, the FIA proved be sensitive economic analytical tool PHE. Within concentration range 5-300 μg.mL-1, calibration curve was rectilinear, where detection limit 3.252 RSD% 4.09. Recovery 100.55% sample throughput 62 sample/hr. proposed successfully applied determining serum samples pharmaceutical formulations, interference excipients studied. mechanism chemical has been proposed. When suggested method's results were compared those provided by British Pharmacopeia reference method, there no discernible difference between two 95 percent confidence level.